Antecedent events underlying axon damage in an animal model of multiple sclerosis

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dc.contributor.advisor Bishop, Derron L. Brinkoetter, Mary T. en_US 2011-06-09T15:32:20Z 2011-06-09T15:32:20Z 2009 en_US 2009
dc.description.abstract Multiple sclerosis is a progressive autoimmune disease where myelin is gradually stripped from axons. Axon degeneration inevitably follows protracted myelin loss ultimately leading to irreversible neurological decline. To better understand the cellular mechanisms associated with the axon loss phase of the disease, spinal cord axons from the experimental autoimmune encephalomyelitis (EAE) animal model of multiple sclerosis were examined using correlated in vivo time-lapse microscopy and serial section transmission electron microscopic (ssTEM) reconstruction. A novel technique, termed near infrared burning (NIRB), was developed that took advantage of a femtosecond-pulsed mode locked laser’s ability to create photoconvertable fiducial markers for routine identification of previously imaged axons for ssTEM reconstruction. This combination of imaging techniques revealed the subcellular milieu that underlies axon degeneration at both the light and electron microscopic level. In particular, paranodal regions of axons in EAE animals contained a significantly higher population of mitochondria with large rounded, electron lucid, vesiculated mitochondria with unorganized cristae compared to controls. This effect was largely restricted to the paranodal region and was not always associated with direct immune cell interaction or myelin loss. Together, these results suggest a novel mechanism for axon degeneration that is not only focal in nature, but decoupled with myelin loss in the EAE animal model of multiple sclerosis.
dc.description.sponsorship Department of Biology
dc.format.extent v, 47 p. : digital, PDF file, ill. (some col.). en_US
dc.source CardinalScholar 1.0 en_US
dc.subject.lcsh Axons en_US
dc.subject.lcsh Demyelination
dc.subject.lcsh Multiple sclerosis--Animal models
dc.subject.lcsh Transmission electron microscopy
dc.subject.lcsh Multiple sclerosis--Imaging
dc.title Antecedent events underlying axon damage in an animal model of multiple sclerosis en_US Thesis (M.S.)
dc.identifier.cardcat-url en_US

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  • Master's Theses [5318]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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