Comparison of the anti-basal ganglia and anti-phospholipid properties of mAb10F5 and IgG2 subtype controls

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dc.contributor.advisor Kelly-Worden, Marie L.
dc.contributor.author Osborne, Mathew S.
dc.date.accessioned 2011-08-12T17:33:35Z
dc.date.available 2011-08-13T05:30:14Z
dc.date.created 2011-07-23
dc.date.issued 2011-07-23
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/123456789/194971
dc.description Access to thesis permanently restricted to Ball State community only
dc.description.abstract Group A streptococcal disorders can result from autoantibodies generated against M proteins. These autoantibodies cross react with the basal ganglia resulting in movement disorders. Previously, we demonstrated binding of streptococcal mAb10F5, with CPu and phospholipids. To determine if mAb10F5 binding to basal ganglia and phospholipids is due to virulence of the antibody or antibody subtype, rats were injected with control IgG2 antibodies and euthanized after 24, 48, or 72 hours. Brains were harvested and immunofluorescence was used to analyze brain slices. Control IgG2 rats showed significantly less fluorescence in the CPu than mAb10F5 injected rats at every time point. These findings reaffirm 10F5 is an anti-basal ganglia antibody. To evaluate mechanism of antibody entry, mAb10F5 was examined for anti-phospholipid activity. MAb10F5 displayed greater affinity to phospholipids when compared to IgG2 controls. Our findings support mAb10F5 is an anti-basal ganglia and anti-phospholipid antibody due to its own virulence.
dc.description.sponsorship Department of Physiology and Health Science
dc.subject.lcsh Streptococcal infections--Immunological aspects
dc.subject.lcsh Phospholipid antibodies
dc.subject.lcsh Bacterial proteins
dc.subject.lcsh Immunoglobulin G.
dc.subject.lcsh Basal ganglia--Diseases
dc.title Comparison of the anti-basal ganglia and anti-phospholipid properties of mAb10F5 and IgG2 subtype controls en_US
dc.description.degree Thesis (M.S.)
dc.date.liftdate 2011-08-13
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1657740


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  • Master's Theses [5293]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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