Examination of hybrid mouse meiotic metaphase spreads with MLH3 and MUS81 resolvase activity

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dc.contributor.advisor Blakey, C. Ann
dc.contributor.author Gribbell, Mikalah A.
dc.date.accessioned 2017-07-31T15:34:26Z
dc.date.available 2017-07-31T15:34:26Z
dc.date.issued 2017-05
dc.identifier.other A-379
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/123456789/200864
dc.description.abstract Mouse meiosis is used as a model for how DNA double strand breaks (DSBs) that have been stabilized into double Holliday Junctions (dHJs) can be resolved. In purebred mouse lines, information on recombination frequencies in the presence or absence of DNA dHJ resolvases has already been published, but questions lingered about the dHJ resolvase protein activity in hybrid mice. The tested hypothesis was that the presence or absence of DNA dHJ resolvase activity in hybrid mice would have the same affect on recombination frequencies as in purebred mouse lines. Bright-field microscopy and macrophotography of chromosomes from B6xDBA F1 hybrid MLH3 and MUS81 Heterozygote (Het) and Knockout (KO) mouse line primary spermatocytes in metaphase I of prophase I of meiosis was used to visualize bivalent and univalent formation as an indication of the affects of the resolvase activity, or lack thereof. Analysis of nearly 200 cells from four different mouse genotypes revealed that the hybrid mice behave the same as the purebred mice with respect to dHJ resolvase activity. In addition, an aberrant chromosome translocation was discovered in the MUS81 hybrid mouse line. en_US
dc.description.sponsorship Honors College
dc.subject.lcsh Biology.
dc.title Examination of hybrid mouse meiotic metaphase spreads with MLH3 and MUS81 resolvase activity en_US
dc.type Undergraduate senior honors thesis.
dc.description.degree Thesis (B.?) en_US
dc.identifier.cardcat-url http://liblink.bsu.edu/uhtbin/catkey/1851894


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  • Undergraduate Honors Theses [5463]
    Honors theses submitted to the Honors College by Ball State University undergraduate students in partial fulfillment of degree requirements.

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