Tumoricidal activity of pulmonary alveolar macrophages isolated from C57BL/6 mice bearing either a cloned metastatic or nonmetastatic variant of Lewis lung carcinoma

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dc.contributor.advisor Higgins, A. Stephen (Arthur Stephen) en_US
dc.contributor.author Duffie, Gordon Patrick en_US
dc.date.accessioned 2011-06-03T19:25:02Z
dc.date.available 2011-06-03T19:25:02Z
dc.date.created 1988 en_US
dc.date.issued 1988
dc.identifier LD2489.Z64 1988 .D84 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/175944
dc.description.abstract The spontaneous tumoricidal ability of pulmonary alveolar macrophages (PAM) isolated from C57B1/6 mice bearing either a metastatic or a nonmetastatic cloned variant of Lewis lung carcinoma (LLC) was examined in vitro. During the early weeks of tumor development the cytotoxicity mediated by macrophages was enhanced in the tumor-bearing mice, especially in the metastatic tumor bearers. Later in tumor progress (week 4) the spontaneous cytotoxicity of both groups typically declined to levels less than those of normal macrophages. Experiments were performed to determine if macrophages could be activated further in vitro by incubation in a mixture of lymphokine and lipopolysaccha ride. The macrophages from the metastatic tumor bearers were consistently activated in vitro. However, macrophages isolated from mice bearing large tumors and whose spontaneous cytotoxicity was suppressed could not be activated.The secretion of prostaglandin E2 (PGE2) by macrophages at different times during tumor development was measured to determine if PGE2 levels corresponded with the ability or inability of macrophages to kill tumor cells. Secretion of PGE2 typically corresponded with the capacity to kill rather than with an inability to kill target cells. Similarly, the production of PGE2 by macrophages was not responsible for the decline in the ability of macrophages to kill tumor cells.These results suggest that PAM are activated to be cytotoxic during the period when pulmonary metastases are developing. The successful establishment of these metastases does not appear to depend on the capacity of the tumor to suppress alveolar macrophage cytotoxicity. en_US
dc.description.sponsorship Department of Biology
dc.format.extent x, 46 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Macrophages. en_US
dc.subject.lcsh Pulmonary alveoli. en_US
dc.subject.lcsh Tumors. en_US
dc.subject.lcsh Tumors -- Growth. en_US
dc.subject.lcsh Metastasis. en_US
dc.subject.lcsh Lungs -- Cancer. en_US
dc.title Tumoricidal activity of pulmonary alveolar macrophages isolated from C57BL/6 mice bearing either a cloned metastatic or nonmetastatic variant of Lewis lung carcinoma en_US
dc.description.degree Thesis (D. Ed.) en_US
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/558376 en_US


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  • Doctoral Dissertations [3121]
    Doctoral dissertations submitted to the Graduate School by Ball State University doctoral candidates in partial fulfillment of degree requirements.

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