The fatty acid desaturase system of Penicillium chrysogenum

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dc.contributor.advisor Bennett, Alice S. en_US Mills, Verne M., 1944- en_US 2011-06-03T19:30:19Z 2011-06-03T19:30:19Z 1970 en_US 1970
dc.identifier LD2489.Z78 1970 .M5 en_US
dc.description.abstract The conversion of stearic acid and lauric acid to unsaturated fatty acids by the desaturase system of Penicillium chrysogenum was investigated by incubating cell free fractions prepared from mycelial sonicates with the radioactively labeled fatty acids and analyzing the reaction products. In some experiments exogenous acyl carrier protein (ACP) or coenzyme A (Co.A) was added to the incubation mixtures. The percentage of radioactive label appearing in unsaturated fractions was regarded as a measure of the activity of the desaturase system.Desaturase activity was detected in both the 15,000 x g particulate and supernatant fractions. Only small percentages of 1-14C-stearyl-CoA were converted to unsaturated fatty acids; 1-14Clauryl-CoA was apparently more readily desaturated. The desaturase system was found to be inhibited by exogenous ACP, but not by exogenous CoA. This suggested that the fatty aryl-CoA thioesters were converted to thioesters of ACP prior to desaturation. en_US
dc.format.extent ii, 25 leaves ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Penicillium chrysogenum. en_US
dc.subject.lcsh Fatty acids. en_US
dc.title The fatty acid desaturase system of Penicillium chrysogenum en_US Thesis (M.S.) en_US
dc.identifier.cardcat-url en_US

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  • Master's Theses [5318]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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