The effects of diflubenzuron on the growth characteristics of chitinase producing bacteria

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dc.contributor.advisor Warnes, Carl E. en_US Glossenger, Daniel H. en_US 2011-06-03T19:35:22Z 2011-06-03T19:35:22Z 1990 en_US 1990
dc.identifier LD2489.Z78 1990 .G56 en_US
dc.description.abstract The effects of the insecticide diflubenzuron (DFB) on the growth characteristics of two gram negative soil bacterial isolates were examined. The bacterial isolates were grown in the presences of varying amounts of DFB and showed no apparent change in cell numbers, over time, when compared to control cultures. However, one of the two isolates tested showed a delayed response in chitinase production, over the same time period, as determined by radioisotopic methodology. Chitinase fractions obtained from cell free culture supernatants, concentrated by ultrafiltration and purified by affinity chromatography, were examined for activity in the presence of DFB. High performance liquid chromatography (HPLC) techniques were utilized in the identification and quantification of products from control and experimental samples. Due to the loss of overall chitinase activity in the supernatants, this technique proved ineffective in determining chitinase response to DFB.
dc.description.sponsorship Department of Biology
dc.format.extent 41 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Diflubenzuron. en_US
dc.subject.lcsh Chitin. en_US
dc.subject.lcsh Pesticides -- Environmental aspects. en_US
dc.subject.lcsh Soil microbiology. en_US
dc.title The effects of diflubenzuron on the growth characteristics of chitinase producing bacteria en_US Thesis (M.S.)
dc.identifier.cardcat-url en_US

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  • Master's Theses [5318]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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