Degradation of atrazine by soil consortia : characterization of enzymatically active fractions from cell bound and cell free enrichment cultures

Cardinal Scholar

Show simple item record

dc.contributor.advisor Warnes, Carl E. en_US
dc.contributor.author Maleki, Saber H. en_US
dc.date.accessioned 2011-06-03T19:37:51Z
dc.date.available 2011-06-03T19:37:51Z
dc.date.created 1997 en_US
dc.date.issued 1997
dc.identifier LD2489.Z78 1997 .M35 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/185872
dc.description.abstract Soil samples were collected from several corn fields with history of atrazine (herbicide) application. Samples were inoculated into Erlenmeyer flasks each containing 50m1 of minimal basal salts medium amended with 100 ppm atrazine as sole nitrogen source. Flasks were shaken at 200 rpm at ambient temperature and were examined daily for one week for microbial growth and/or disappearance of atrazine. Promising consortia were subcultured for further additional enrichments before characterization of potentially active protein (enzyme)fractions. Proteins from cell-free and cellbound fractions were compared for ability to denature atrazine. Following gel permeation chromatography, isolated protein fractions were examined for atrazinefound in the cell-bound fractions capable degrading degradation. Two were found in the cell-free fractions (approx. Mol. wts. 55kDa and 180kDa) and one (55 kDa) atrazine to hydroxyatrazine. Atrazine and its breakdown products (hydroxyatrazine in particular) were detected via HPLC using C18 and C8 columns with absorbance at 229 nm.
dc.description.sponsorship Department of Biology
dc.format.extent v, 59 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Atrazine. en_US
dc.subject.lcsh Herbicides -- Biodegradation. en_US
dc.subject.lcsh Soils -- Herbicide content. en_US
dc.title Degradation of atrazine by soil consortia : characterization of enzymatically active fractions from cell bound and cell free enrichment cultures en_US
dc.description.degree Thesis (M.S.)
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1048376 en_US


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

  • Master's Theses [5318]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

Show simple item record

Search Cardinal Scholar


Browse

My Account