Legionella pneumophila occurrence in waters of east central Indiana

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dc.contributor.advisor Warnes, Carl E. en_US
dc.contributor.author Troxell, Stephen B. en_US
dc.coverage.spatial n-us-in en_US
dc.date.accessioned 2011-06-03T19:40:39Z
dc.date.available 2011-06-03T19:40:39Z
dc.date.created 2005 en_US
dc.date.issued 2005
dc.identifier LD2489.Z78 2005 .T76 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/187986
dc.description.abstract Drinking water is a potential means for transmission of the opportunistic pathogen Legionella pneumophila. The objective of this research was to document the occurrence of L. pneumophila from source water, partially treated water after conventional water treatment, and distribution water. Water samples were examined for L. pneumophila by direct fluorescent antibody (DFA) techniques and by SYBR Green based real-time multiplex PCR. Primers were designed to amplify a 16S product of 490 bp and a mip product of 290 bp within L. pneumophila. Sensitivity of culture methods and PCR was determined by percent recovery and by using serial dilutions of positive control DNA, respectively. Eighty percent of source water samples were positive for L. pneumophila by real-time PCR versus 100% positive by DFA for L. pneumophila. Twenty percent of GAC filter water samples were positive for L. pneumophila by real-time PCR versus 40% positive by DFA. Distribution water samples yielded mixed results. Twenty percent of biofilms from the distribution system were positive for L. pneumophila by real-time PCR. This project confirms the potential for human infection by L. pneumophila from distribution water within the study area.
dc.description.sponsorship Department of Biology
dc.format.extent iii, 64 leaves : ill. (some col.) ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Legionella pneumophila -- Indiana -- Muncie -- Analysis. en_US
dc.subject.lcsh Bacterial pollution of water -- Indiana -- Muncie. en_US
dc.title Legionella pneumophila occurrence in waters of east central Indiana en_US
dc.description.degree Thesis (M.S.)
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1319834 en_US

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  • Master's Theses [5318]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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