Oxytricha cyst cDNA library analysis and RNAi examination : an honors thesis (HONRS 499)

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dc.contributor.advisor Hammersmith, Robert L. en_US
dc.contributor.author Kane, Daniel P. en_US
dc.date.accessioned 2011-06-06T19:03:20Z
dc.date.available 2011-06-06T19:03:20Z
dc.date.created 2006 en_US
dc.date.issued 2006
dc.identifier.other A-308 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/191082
dc.description.abstract Oxytricha trifallax is a fascinating organism for genetic study. This ciliate undergoes precise disassembly and reassembly of ciliary structures during several different developmental processes by mechanisms not well defined or understood. Understanding genetic mechanisms behind intracellular reorganization is possible by creating cDNA libraries and Finding cDNAs specific to certain stages of development. It is the goal of the laboratory to create these cDNA libraries for multiple developmental processes and stages. But before that can occur, the protocol needs to be analyzed for its feasibility and all troubleshooting worked out. It is one aim of this thesis to analyze the data from a cyst cDNA library to determine what may need to be altered for future library construction. The second aim of this thesis is to determine the feasibility of another method for analyzing genes important to specific stages of development. With the increased interest in RNA interference, this thesis explores the possibility of using dsRNA at the small scale, single-cell level to interfere with gene expression for purposes of analyzing genes found to be important to developmental stages through library creation.
dc.description.sponsorship Honors College
dc.format.extent 23 leaves : ill. ; 30 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Biology. en_US
dc.title Oxytricha cyst cDNA library analysis and RNAi examination : an honors thesis (HONRS 499) en_US
dc.type Undergraduate senior honors thesis
dc.description.degree Thesis (B.?.)
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1338971 en_US

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  • Undergraduate Honors Theses [5596]
    Honors theses submitted to the Honors College by Ball State University undergraduate students in partial fulfillment of degree requirements.

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