Extraction of potential chemical attractants from Rudbeckia hirta inflorescences

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dc.contributor.advisor Lang, Patricia L.
dc.contributor.author Judkins, Rojenia N. en_US
dc.date.accessioned 2011-06-09T15:32:27Z
dc.date.available 2011-06-09T15:32:27Z
dc.date.created 2009 en_US
dc.date.issued 2009
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/123456789/193488
dc.description.abstract We aimed to identify the volatile compounds in inflorescences of Rudbeckia hirta that may be responsible for the olfactory attraction of the crab spider Misumenoides formosipes to this plant. Our approach was to use ultrasonic extraction, separate the extract into fractions using flash chromatography with different solvent systems, and test the attraction of the male spiders to the pooled fractions using a y-tube olfactometer. Ultrasonic extraction is carried out using a mixture of 1:2 hexane/diethyl ether with 10 g of inflorescences for 30 minutes. Bioassay results indicated that male spiders chose the inflorescences, bulk ultrasonic extract, and the pooled 100% dichloromethane fractions over controls. Nuclear magnetic resonance experiments and infrared spectroscopy experiments were carried out on the 100% dichloromethane fractions and these experiments indicated that a long chain hydrocarbon is the main component in the 100% dichloromethane fractions
dc.description.sponsorship Department of Chemistry
dc.description.tableofcontents Chromatographic method and bioassay development method -- M. formosipes olfactory response to R. hirta -- Separation and identification of the possible attractants in the 100% dichloromethane fractions.
dc.format.extent ix, 56 p. : digital, PDF file, ill. (some col). en_US
dc.source CardinalScholar 1.0 en_US
dc.subject.lcsh Volatile organic compounds -- Analysis. en_US
dc.subject.lcsh Crab spiders -- Sexual behavior.
dc.subject.lcsh Rudbeckia -- Morphology.
dc.title Extraction of potential chemical attractants from Rudbeckia hirta inflorescences en_US
dc.description.degree Thesis (M.S.)
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1503983 en_US


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  • Master's Theses [5318]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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