A novel method of assessing human skeletal muscle fiber type specific protein content

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dc.contributor.advisor Trappe, Scott William
dc.contributor.author Galpin, Andrew J.
dc.date.accessioned 2011-08-04T18:59:23Z
dc.date.available 2011-08-05T05:30:05Z
dc.date.created 2011-07-23
dc.date.issued 2011-07-23
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/123456789/194891
dc.description Access to thesis permanently restricted to Ball State community only
dc.description.abstract Little is known about protein profiles in slow-twitch (MHC I) and fast-twitch (MHC IIa and MHC IIx) human skeletal muscle fibers. Therefore we developed a method of assessing fiber type specific protein content across the continuum of human skeletal muscle fiber types. The method presented here combines the advantages of SDS-PAGE for fiber typing with the common Western Blot (WB) technique. Individual vastus lateralis muscle fibers (n = 264) were isolated and clipped into two portions, one for fiber-typing and one for protein identification. Following fiber type determination, WB destined fiber segments were combined into fiber type specific pools (20 fibers/pool) and assessed for GAPDH, actin, Citrate Synthase, and total p38 content. GAPDH expression was 69%, 92%, 159%, and 200% more abundant in MHC I/IIa, MHC IIa, MHC IIa/IIx, and MHC IIx pools when compared to MHC I, respectively. Inversely, Citrate synthase content was 526%, 497%, 316%, and 47% more abundant in MHC I, MHC I/IIa, MHC IIa, and MHC IIa/IIx when compared to MHC IIx, respectively. Similar to GAPDH, total p38 expression was 67% greater in MHC IIa versus MHC I fibers. These data establish a novel application of WB combined with SDS-PAGE for fiber type specific protein analysis in human skeletal muscle. These initial results show content of particular proteins exist in a hierarchal fashion throughout the continuum of human skeletal muscle fiber types. Application of these methods will enhance our understanding of skeletal muscle health profiles among physically active and clinically based populations. en_US
dc.description.sponsorship School of Physical Education, Sport, and Exercise Science
dc.subject.lcsh Skeleton -- Muscles -- Physiology.
dc.subject.lcsh Muscle proteins -- Composition.
dc.subject.lcsh Vastus lateralis -- Physiology.
dc.title A novel method of assessing human skeletal muscle fiber type specific protein content en_US
dc.description.degree Thesis (Ph. D.) en_US
dc.date.liftdate 2011-08-05
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1652231

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  • Doctoral Dissertations [3286]
    Doctoral dissertations submitted to the Graduate School by Ball State University doctoral candidates in partial fulfillment of degree requirements.

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