Disruption of esophageal tissue hinders oral tolerance induction to ovalbumin

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dc.contributor.advisor Bruns, Heather A.
dc.contributor.author Kinder, Jeremy M.
dc.date.accessioned 2012-05-22T19:12:48Z
dc.date.available 2013-12-26T20:17:46Z
dc.date.created 2012-05-05
dc.date.issued 2012-05-05
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/123456789/195900
dc.description.abstract Previous data in our lab demonstrated an inability to induce oral tolerance when using a feeding needle gavage for 14 days. Given that the upper gastrointestinal (GI) tract is the site of antigen introduction, and the interplay between immune cells of the mucosal tissues, we questioned if inflammation in this tissue, induced by physical trauma, would affect oral tolerance induction. We performed studies on Balb/c mice using a needle gavage or syringe feeding method followed by doses of the immunogenic protein ovalbumin (OVA) to induce tolerance. Immunohistochemistry was used to assess inflammation in esophageal tissues and to correlate with an ability or inability to induce tolerance. Non-cellular alterations within the tissue were also assessed using a pathology grading score. Although fluctuations in cell populations were observed in both the syringe and gavage treated mice, the needle gavage caused significant noncellular damage to esophageal mucosal tissue, which is the most likely cause of failed tolerance induction to the OVA.
dc.description.sponsorship Department of Biology
dc.subject.lcsh Tube feeding -- Physiological aspects.
dc.subject.lcsh Esophagus -- Physiology.
dc.subject.lcsh Gastrointestinal mucosa -- Immunology.
dc.subject.lcsh Antigens.
dc.subject.lcsh Immunoglobulin G.
dc.title Disruption of esophageal tissue hinders oral tolerance induction to ovalbumin en_US
dc.title.alternative Title on signature form: Disruption of esophageal tissue hinders oral tolerance induction
dc.description.degree Thesis (M.S.)
dc.date.liftdate 2012-05-23
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1670054

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  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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