The effect of the aqueous extract of Lonicera japonica on antigen-stimulated T cell function

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dc.contributor.advisor Bruns, Heather A. Brooks, Austin D. 2013-05-09T15:54:04Z 2013-05-09T15:54:04Z 2013-05-04 2013-05-04
dc.description.abstract Lonicera japonica is a honeysuckle species commonly used in traditional Chinese medicine to treat diverse ailments such as headache, fever, cough and sore throat. The aqueous fraction of L. japonica has been shown to have a variety of anti-inflammatory properties in addition to L. japonica being hailed as an immune enhancer. Previous studies examining flavonoids, common constituents of L. japonica extracts, have demonstrated that flavonoids have varying abilities to inhibit lymphocyte functions. The goal of these studies was to examine the effect of the aqueous fraction of L. japonica on T cell functions. Purified murine T cells were assessed for proliferation using an MTT assay following stimulation with plate bound anti-CD3 and soluble anti-CD28 antibodies and/or treatment with L. japonica. The expression of CD25, CD95, CD152, and CD178 as well as cell death using propidium iodide staining were analyzed on CD4+ T cells using flow cytometry. An ELISA was used for assessing IL-2 production. Neutralization assays were performed using αCD178 or αCD152 antibodies in determining alterations in T cell death or proliferation. L. japonica inhibited T cell proliferation and reduced CD25 expression on activated T cells, while having no effect on IL-2 production. In naïve and activated CD4+ T cells, L. japonica increased the expression of CD95, CD152, and CD178 in addition to cell death. Neutralization of CD178 or CD152 did not abrogate increased cell death or reduced proliferation resulting from L. japonica treatment. Our results showed that the aqueous extract of L. japonica is inhibitory to T lymphocyte proliferation and potentially occurs through decreased expression of CD25. In addition, L. japonica increased cell death which was independent of upregulated CD95 and CD178 expression. Findings from this work provide insights into the immunomodulatory properties of L. japonica and subsequent effects on T cell responses.
dc.description.sponsorship Department of Biology
dc.subject.lcsh Japanese honeysuckle -- Therapeutic use.
dc.subject.lcsh T cells.
dc.title The effect of the aqueous extract of Lonicera japonica on antigen-stimulated T cell function en_US Thesis (M.S.)

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  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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