Identification of vesicle-associated membrane protein 7 (VAMP7) in rabbit B lymphocytes

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dc.contributor.advisor Kelly-Worden, Marie L. French, Kyleigh Anne 2014-05-06T14:22:58Z 2014-05-06T14:22:58Z 2014-05-03
dc.description Access to thesis permanently restricted to Ball State community. en_US
dc.description.abstract VAMP-7 has been found to interact with SNAP-23, a t-SNARE that functions in relocating granule membranes in response to stimulation, and plays a large role in the regulation of granule release from mast cells in response to an allergic reaction. While evidence suggests that VAMP-7 is active in antibody release in the innate immune system, little investigation has been completed on VAMP-7 interaction in specific antibody release of B lymphocytes of the humoral immune system. Little research has previously focused on vesicular transport within B lymphocytes, leaving molecular mechanisms within B lymphocytes a mystery. Immunodot blots, western blots, and immunoflourescent microscopy were all utilized with the goal of identifying the presence of VAMP- 7. Immunobot blots for both 55D1 and 240E cells were all negative for the presence of VAMP-7. However, VAMP-7 was detected using immunoflourescent microscopy in both 55D1 and 240E cell lines.
dc.description.sponsorship Department of Physiology and Health Science
dc.subject.lcsh Synaptic vesicles.
dc.subject.lcsh Membrane proteins.
dc.subject.lcsh B cells.
dc.title Identification of vesicle-associated membrane protein 7 (VAMP7) in rabbit B lymphocytes en_US
dc.title.alternative Identification of vesicle associated membrane protein 7 (VAMP-7) in rabbit B lymphocytes
dc.title.alternative Title on signature form: Identification of vesicle-associated protein 7 (VAMP-7) in rabbit B lymphocytes Thesis (M.S.)

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  • Master's Theses [5454]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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