Mapping of structural changes to the ykkCD antibiotic sensor RNA caused by tetracycline binding

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dc.contributor.advisor Gerczei, Timea
dc.contributor.author Conley, Caroline Anne
dc.date.accessioned 2015-07-23T13:07:54Z
dc.date.available 2015-07-23T13:07:54Z
dc.date.issued 2015-06-17
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/123456789/199843
dc.description.abstract A riboswitch is a noncoding RNA that controls gene expression response to changes in the cellular environment. Most riboswitches regulate genes responsible for transporting, synthesizing or recycling small metabolites in bacteria.1 These riboswitches, primarily found at the 5’ region of messenger RNA, recognize the metabolic product of the gene being regulated. When a metabolite concentration threshold is reached, expression is turned off. In contrast, the ykkCD putative riboswitch increases production of an efflux pump that ejects toxins from the cell by binding to the antibiotic tetracycline. As tetracycline binds to ykkCD riboswitch, structural changes take place that trigger the production of the efflux pump.2 We will present terbium fragmentation and other nucleic acid footprinting methods to map the tetracycline site and to visualize the allosteric changes that take place due to tetracycline binding. en_US
dc.description.sponsorship Department of Chemistry
dc.subject.lcsh Riboswitches.
dc.subject.lcsh Genetic regulation.
dc.subject.lcsh Drug resistance in microorganisms -- Genetic aspects.
dc.subject.lcsh Tetracycline.
dc.title Mapping of structural changes to the ykkCD antibiotic sensor RNA caused by tetracycline binding en_US
dc.description.degree Thesis (M.S.) en_US
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1800696


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  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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