Investigating the deposition of Streptococcus pyogenes M-6 10F5 epitope antibody production and macrophage infiltration in Lewis rat hearts

Cardinal Scholar

Show simple item record

dc.contributor.advisor Kelly-Worden, Marie L.
dc.contributor.author Cuebas, Victoria L.
dc.date.accessioned 2016-07-27T15:29:31Z
dc.date.available 2016-07-27T15:29:31Z
dc.date.issued 2016-07-23
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/123456789/200310
dc.description Access to thesis permanently restricted to Ball State community only en_US
dc.description.abstract Group A Streptococcus pyogenes (GAS) is responsible for 700 million infections each year. Specific M proteins of GAS have been shown to contribute to autoimmune antibody production. To determine the effects of the M6 10F5 epitope of GAS, this peptide was injected subcutaneously, along with a Stimune adjuvant, into the experimental (EX) group of Lewis rats. Negative control rats receive no injection and experimental control (EC) rats received Stimune only injections. Each injection was given on day 1 and day 28. Hearts were harvested after euthanasia, sectioned into atria, valve, and ventricle regions, and sliced. Immunofluorescence was used to analyze heart slices. A significant amount of antibody binding over EC was observed in only the ventricle region. Macrophage infiltration into the valve was greatest in EX. Libman-Sacks vegetations were observed in 67% of EX heart valves. The 10F5 epitope generated antiphospholipid antibodies that promoted the production of Libman-Sacks endocarditis vegetations. en_US
dc.description.sponsorship Department of Biology
dc.subject.lcsh Bacterial antigens.
dc.subject.lcsh Streptococcus pyogenes.
dc.subject.lcsh Streptococcal infections -- Immunological aspects.
dc.subject.lcsh Rats -- Cardiovascular system -- Diseases.
dc.title Investigating the deposition of Streptococcus pyogenes M-6 10F5 epitope antibody production and macrophage infiltration in Lewis rat hearts en_US
dc.title.alternative Title on signature form: Investigating the deposition of Streptococcus pyogens M-6 10F5 epitope antibody production and macrophage infiltration in Lewis rat hearts
dc.description.degree Thesis (M.S.) en_US
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1825307


Files in this item

This item appears in the following Collection(s)

  • Master's Theses [5454]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

Show simple item record

Search Cardinal Scholar


Browse

My Account