Isolation and Characterization of Halotolerant 2,4- Dichlorophenoxyacetic Acid Degrading Bacteria From Sulfidic, Low Salinity Salt Springs

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dc.contributor.author Willis, Michael G.
dc.contributor.author Treves, David S.
dc.date.accessioned 2018-08-17T14:34:35Z
dc.date.available 2018-08-17T14:34:35Z
dc.date.issued 2014
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/123456789/201345
dc.description.abstract The bacterial communities at two sulfidic, low salinity springs with no history of herbicide contamination were screened for their ability to grow on 2,4-dichlorophenoxyacetic acid (2,4-D). Nineteen isolates, closely matching the genera Bacillus, Halobacillus, Halomonas, Georgenia and Kocuria, showed diverse growth strategies on NaCl-supplemented and NaCl-free 2,4-D medium. The majority of isolates were halotolerant, growing best on nutrient rich broth with 0% or 5% NaCl; none of the isolates thrived in medium with 20% NaCl. The tfdA gene, which codes for an a – ketoglutarate dioxygenase and catalyzes the first step in 2,4-D degradation, was detected in nine of the salt spring isolates. The tfdAa gene, which shows ~60% identity to tfdA, was present in all nineteen isolates. Many of the bacteria described here were not previously associated with 2,4-D degradation suggesting these salt springs may contain microbial communities of interest for bioremediation. en_US
dc.subject 2,4-D en_US
dc.subject tfdA en_US
dc.subject tfdAa en_US
dc.subject salt springs en_US
dc.subject halotolerant bacteria en_US
dc.title Isolation and Characterization of Halotolerant 2,4- Dichlorophenoxyacetic Acid Degrading Bacteria From Sulfidic, Low Salinity Salt Springs en_US
dc.type Article en_US


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  • Fine Focus journal [54]
    Fine Focus, an international microbiology journal for undergraduate research

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