The development of CRISPR-mediated genetic engineering in Candida viswanathii

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dc.contributor.advisor Bernstein, Douglas A., 1979- North, Elena 2020-11-19T19:11:17Z 2020-11-19T19:11:17Z 2020-05
dc.description.abstract Candida viswanathii is a fungus that efficiently produces long-chain dicarboxylic acids, which are useful in petrochemical production. It is closely related to Candida tropicalis, a prominent human fungal pathogen. Because CRISPR has been implemented in other Candida species, particularly Candida albicans, I hypothesized that a similar CRISPR-mediated genome editing method could be developed for C. viswanathii. I have sequenced the C. viswanathii genome and identified guide sequences for ADE2 and CYP52. I then cloned these sequences into a vector that also expresses the Cas9 nuclease. I cotransformed these vectors along with a repair template that will introduce stop codons when incorporated into ADE2 and CYP52 of C. viswanathii. I was able to obtain many transformants, and I am now determining if these transformants have incorporated the repair template. My ultimate goal is to work in collaboration to determine why and how C. viswanathii makes long-chain dicarboxylic acids en_US
dc.description.sponsorship Honors College
dc.title The development of CRISPR-mediated genetic engineering in Candida viswanathii en_US
dc.type Undergraduate senior honors thesis Thesis (B.?) en_US

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  • Undergraduate Honors Theses [5928]
    Honors theses submitted to the Honors College by Ball State University undergraduate students in partial fulfillment of degree requirements.

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