The development of CRISPR-mediated genetic engineering in Candida viswanathii

Cardinal Scholar

Show simple item record

dc.contributor.advisor Bernstein, Douglas A., 1979-
dc.contributor.author North, Elena
dc.date.accessioned 2020-11-19T19:11:17Z
dc.date.available 2020-11-19T19:11:17Z
dc.date.issued 2020-05
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/123456789/202620
dc.description.abstract Candida viswanathii is a fungus that efficiently produces long-chain dicarboxylic acids, which are useful in petrochemical production. It is closely related to Candida tropicalis, a prominent human fungal pathogen. Because CRISPR has been implemented in other Candida species, particularly Candida albicans, I hypothesized that a similar CRISPR-mediated genome editing method could be developed for C. viswanathii. I have sequenced the C. viswanathii genome and identified guide sequences for ADE2 and CYP52. I then cloned these sequences into a vector that also expresses the Cas9 nuclease. I cotransformed these vectors along with a repair template that will introduce stop codons when incorporated into ADE2 and CYP52 of C. viswanathii. I was able to obtain many transformants, and I am now determining if these transformants have incorporated the repair template. My ultimate goal is to work in collaboration to determine why and how C. viswanathii makes long-chain dicarboxylic acids en_US
dc.description.sponsorship Honors College
dc.title The development of CRISPR-mediated genetic engineering in Candida viswanathii en_US
dc.type Undergraduate senior honors thesis
dc.description.degree Thesis (B.?) en_US


Files in this item

This item appears in the following Collection(s)

  • Undergraduate Honors Theses [5928]
    Honors theses submitted to the Honors College by Ball State University undergraduate students in partial fulfillment of degree requirements.

Show simple item record

Search Cardinal Scholar


Browse

My Account