Abstract:
Vladimir Skipski from the Memorial Sloan-Kettering Cancer Center in New York has isolated two neoproteolipids in Walker carcinosarcomas from rats. In our laboratory, we have insured that these neoproteolipids are present in mouse mammary adenocarcinomas. The sub-cellular location of neoproteolipids in spontaneous mouse mammary adenocarcinomas was identified.Tumor tissue was separated into plasma membrane, mitochondrial, and microsomal-soluble fractions by differential centrifugation. Lipids were extracted from each fraction. By using thin-layer chromatography fractions which contained these neoproteolipids were identified.Neoproteolipids were detected in plasma membrane and mitochondria) fractions. No microsomal fraction showed the presence of neoproteolipids. Several normal mammary tissue fractions tested also showed a negative result for these lipids.