The incorporation of carbon-14 labeled fatty acids into cellular components of Bacillus thuringiensis and Escherichia coli

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dc.contributor.advisor Bennett, Alice S. en_US
dc.contributor.author Kindig, Charles R., 1940- en_US
dc.date.accessioned 2011-06-03T19:30:10Z
dc.date.available 2011-06-03T19:30:10Z
dc.date.created 1970 en_US
dc.date.issued 1970
dc.identifier LD2489.Z78 1970 .K5 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/179867
dc.description.abstract Bacillus thuringiensis and .:scherichia coli were grown in submerged cultures in the presence of 1-014 labeled decanoic, palmitic and oleic acids and 2-C14 labeled malonic acid. The bacteria were harvested by centrifugation and fractionated by published methods. Cellular constituents were separated and identified by chromatographic techniques. Radioactivity was determined by liquid scintillation spectrometry and through the use of a radiochromatogram scanner.The bacteria incorporated all the fatty acids added to the medium but showed a preference for the longer chained compounds. The organisms incorporated 1-014 palmitic acid into mostly phospholipids and also into free fatty acids and neutral lipids. At least 90% of the incorporated palmitate remained as palmitic acid. Elongation to stearic acid was demonstrated by B. thuringiensis. The label from 1-014 palmitic acid was retained over several generations by both test organisms.Ball State UniversityMuncie, IN 47306 en_US
dc.format.extent iii, 31 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Bacillus thuringiensis. en_US
dc.subject.lcsh Escherichia coli. en_US
dc.subject.lcsh Fatty acids -- Metabolism. en_US
dc.subject.other Ball State University. Thesis (M.S.) en_US
dc.title The incorporation of carbon-14 labeled fatty acids into cellular components of Bacillus thuringiensis and Escherichia coli en_US
dc.description.degree Thesis (M.S.)--Ball State University, 1970. en_US
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/416062 en_US


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  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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