The feasibility of using calcofluor white as a fluorescent tracer for the rapid screening of bacterial establishment in Tenebrio molitor Linnaeus

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dc.contributor.advisor Zimmack, Harold L. en_US
dc.contributor.author Rice, David T., 1947- en_US
dc.date.accessioned 2011-06-03T19:30:38Z
dc.date.available 2011-06-03T19:30:38Z
dc.date.created 1971 en_US
dc.date.issued 1971
dc.identifier LD2489.Z78 1971 .R53 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/180378
dc.description.abstract AbstractFive bacteria, Escherichia coli, Serratia marcescens, Bacillus subtilis,, Bacillus cereus, and Sarcina flava, were tested for establishment in the yellow mealworm, Tenebrio molitor, using a fluorescent screening technique. Through literature research some of the above bacteria were known to establish in the mealworm.The various test bacteria were dyed with Calcofluor white, a fluorescent dye, and introduced to the mealworm by several techniques. Oral injection, anal injection, drop on head, and petri dish methods were attempted. The best method of bacterial entry into the insect was found to be the drop on head. Since few bacteria were ingested by the insect, a quantitative analysis was impossible.It was found that as the dyed bacteria grew, fluorescence decreased. This was substantiated by allowing dyed Sarcina flava to grow at 37 degrees C. in nutrient broth and examining every hour for ten hours.Although this study did not show a definite screening technique for bacterial establishment in Tenebrio molitor Linnaeus, the results from this project could aid researchers in developing such a technique.Ball State UniversityMuncie, IN 47306 en_US
dc.format.extent vii, 48 leaves ; ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Tenebrio moliter. en_US
dc.subject.lcsh Pathogenic microorganisms. en_US
dc.subject.lcsh Fluorescence microscopy. en_US
dc.subject.other Ball State University. Thesis (M.S.) en_US
dc.title The feasibility of using calcofluor white as a fluorescent tracer for the rapid screening of bacterial establishment in Tenebrio molitor Linnaeus en_US
dc.description.degree Thesis (M.S.)--Ball State University, 1971. en_US
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/416725 en_US


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  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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