A kinetic study of fatty acid desaturation in penicillium chrysogenum

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dc.contributor.advisor Bennett, Alice S. en_US
dc.contributor.author Chamberlin, Paul T. en_US
dc.date.accessioned 2011-06-03T19:30:44Z
dc.date.available 2011-06-03T19:30:44Z
dc.date.created 1971 en_US
dc.date.issued 1971
dc.identifier LD2489.Z78 1971 .C52 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/180446
dc.description.abstract The effects of oxygen, malonate, and acetate on the conversion of radioactively-labeled Coenzyme A thioesters of lauric and stearic acids to unsaturated fatty acids by 15,000 x g supernates of Penicilliumlehrysogenum were studied. Following the termination of reactions, the incubation products were saponified, acidified, extracted, and methylated. The resulting methyl esters were purified, separated, identified, and collected. Radioactivities of each fatty acid fraction were determined and converted to percentage of total radioactivity recovered.Desaturase activity was virtually non-existent in the anaerobic incubations indicating that this organism utilized the aerobic pathway of desaturation. The absence of acetate and malonate from incubation mixtures of either laurate or stearate markedly decreased the overall desaturase activity and the amount of linoleate produced. The failure of labeled laurate to be converted to long-chain unsaturated fatty acids in the absence of malonate indicates that chainelongation in this organism proceeds by the malonate pathway. The data presented suggest that laurate may be directly desaturated and then elongated to oleate instead of following the typical aerobic pathway.
dc.description.sponsorship Department of Biology
dc.format.extent iii, 38 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Fatty acids -- Synthesis. en_US
dc.subject.lcsh Single cell lipids. en_US
dc.subject.lcsh Penicillium chrysogenum. en_US
dc.title A kinetic study of fatty acid desaturation in penicillium chrysogenum en_US
dc.description.degree Thesis (M.S.)
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/865953 en_US


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  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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