Abstract:
The irreversible binding of penicillin to serum protein was investigated by incubating 14C-benzyl-penicillin with reconstituted lyophilized human serum and dialyzing away the non-bound penicillin. Binding appeared in each protein fraction as was observed by polyacrylamide gel electrophoresis and liquid scintillation counting.The nature of penicillin binding was investigated by preincubating reconstituted human serum with specicific blocking reagents.Ball State UniversityMuncie, IN 47306