Abstract:
Benzylpenicillin, a-aminobenzylpenicillin, phenoxymethylpenicillin, and 2,6-dimethoxy-phenylpenicillin were incubated with whole human blood. Migration patterns into human erythrocytes were compared spectrophotometrically.A possible inhibition effect by chloroquine on benzyl-[14C]-penicillin in red blood cells was studied via a Beckman LS-100C Liquid Scintillation Counter.An intraerythrocytic protein, carbonic anhydrase, was investigated as being responsible for the hydrolysis of benzylpenicillin. Three carbonic anhydrases (a, b, and c) were quantitatively measured via a Beckman IR 4250 Spectrophotometer for their effect upon the a-lactam ring of benzylpenicillin.Ball State UniversityMuncie, IN 47306