Effect of dietary cholesterol on cholesterol synthesis and plasma membrane lipids of mouse mammary adenocarcinomas and mammary gland tissue

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dc.contributor.advisor Bennett, Alice S. en_US
dc.contributor.author Alexander, Lee H. en_US
dc.date.accessioned 2011-06-03T19:33:05Z
dc.date.available 2011-06-03T19:33:05Z
dc.date.created 1981 en_US
dc.date.issued 1981
dc.identifier LD2489.Z78 1981 .A53 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/182463
dc.description.abstract The purpose of this research was to determine if a high cholesterol diet can affect tie rate of cholesterol synthesis or to cholesterol and fatty acid content of plasma membranes of mouse mammary a.denocarcinoma and normal mouse mammary gland tissue.Cholesterol synthesizing ability was determined by measuring the incorporation of 14C acetate into digitonin-precipitable sterols from both tumors and normal mammary tissue from Strong A Strain female mice fed a standard lab chow diet (control diet; or a 2% cholesterol experimental diet. Plasma membranes were isolated from both tenors and normal tissue by differential centrifugation. Cholesterol was measured spectrophotometrically. Fatty acids were extracted, methylated, and methylesters identified and quantified using gas liquid chromatography.The rate of incorporation of 111C acetate into digitonin-precipitated sterols in normal mammary tissue from mice fed the 2% cholesterol experimental diet was 1.5 times less than controls. Tumor tissue showed no significant difference. The fatty acid composition of tumor and normal mammary tissue plasma membranes from mice fed the 2% cholesterol experimental diet was similar to controls.There were higher percentages of C16:0 and C16.1 in normal plasma membranes of controls than mice fed the experimental diet. Cholesterol content of to or and normal mammary tissue plasma membranes from mice fed the experimental diet was similar to controls.It would appear that dietary cholesterol does have an effect on cholesterol synthesis in normal mammary tissue but not in marina y tumor tissue. Also dietary cholesterol does not have an effect on the fatty acid composition nor the cholesterol content in plasma membranes of mammary tumors or normal mammary tissue.Ball State UniversityMuncie, IN 47306 en_US
dc.format.extent v, 46 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Cholesterol -- Metabolism. en_US
dc.subject.lcsh Lipid membranes. en_US
dc.subject.other Ball State University. Thesis (M.S.) en_US
dc.title Effect of dietary cholesterol on cholesterol synthesis and plasma membrane lipids of mouse mammary adenocarcinomas and mammary gland tissue en_US
dc.description.degree Thesis (M.S.)--Ball State University, 1981. en_US
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/460903 en_US


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  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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