High performance liquid chromatography analysis and quantitation of chitinolytic products of CDC group EF-4a

Cardinal Scholar

Show simple item record

dc.contributor.advisor Warnes, Carl E. en_US
dc.contributor.author Baker-Ellis, Judy en_US
dc.date.accessioned 2011-06-03T19:34:03Z
dc.date.available 2011-06-03T19:34:03Z
dc.date.created 1985 en_US
dc.date.issued 1985
dc.identifier LD2489.Z78 1985 .B34 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/183132
dc.description.abstract A chitinolytic organism was cultured from a lotic habitat in central Indiana. It was isolated on the basis of its hydrolytic activity on chitin agar. It was subsequently identified as EF-4a. The chitinases of the EF-4a were isolated, purified and concentrated. A colloidal chitin suspension was then incubated with the enzyme. Samplings were taken at one minute, one hour and 24 hours. The samplings were filtered through a Swinney filtration unit and immediately after applied to a Beckman Ultrasil Amino column. It was eluted with acetonitrile: water (75:25) at a flow rate of 2 ml/min. The absorbance at 214 nm was monitored using the Beckman 160 UV detector. The chromatographs were analyzed for product elaboration. It was observed that EF-4a produced soluble, extracellular enzymes. The enzymes were a chitinase-chitobiase complex. The enzymes' action on chitin was immediate. There were varying reaction products including G1cN, GlcNAc, chitobiose, chitotriose and higher order oligomers. There was also chromatographic evidence suggesting a and B anomers of these saccharides.
dc.format.extent v, 54 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Chitin. en_US
dc.subject.lcsh Chromatographic analysis. en_US
dc.title High performance liquid chromatography analysis and quantitation of chitinolytic products of CDC group EF-4a en_US
dc.description.degree Thesis (M.S.)
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/443555 en_US


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

Show simple item record

Search Cardinal Scholar


Browse

My Account