Autoradiographic localization of pre-messenger RNA in the nucleus of the Necturus maculosus oocyte

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dc.contributor.advisor Ganion, Larry R. en_US Rock, Daniel E. en_US 2011-06-03T19:34:35Z 2011-06-03T19:34:35Z 1987 en_US 1987
dc.identifier LD2489.Z78 1987 .R6 en_US
dc.description.abstract The precise location of pre-messenger RNA (pre-mRNA) was determined within the cell nucleus of the amphibian N. maculosus (mudpuppy) oocyte. Pre-messenger RNA or heterogeneous nuclear RNA (hnRNA) is directly transcribed from the gene in the cell nucleus (Jelinek et al., 1983). This highly unstable, high molecular weight complex is then processed into messenger RNA (mRNA). Again this transformation is thought to take place in the nucleus.By employing the techniques of in situ hybridization along with light and high resolution autoradiography an effort was made to localize, via a radioactive probe, ( 3H) poly (U), pre-mRNA within the nucleus. Additionally, various inhibitors were employed in this study to analyze changes in the amount and distribution of radioactive material within the cell.Light microscopy observations of autoradiographs reveal a consistent pattern of probe localization over the nucleoli with a broader dispersal across the nuclei. Ultrastructural studies reveal the presence of granules (perichromatin granules) localized over both the nucleolar-associated chromatin and at the border of condensed chromatin. These structures are contained in the cell nucleus and are presumed to function in the transport and storage of mRNA.
dc.description.sponsorship Department of Physiology and Health Science
dc.format.extent vii, 57 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Messenger RNA. en_US
dc.subject.lcsh Necturus maculosus. en_US
dc.title Autoradiographic localization of pre-messenger RNA in the nucleus of the Necturus maculosus oocyte en_US Thesis (M.S.)
dc.identifier.cardcat-url en_US

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  • Master's Theses [5454]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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