Construction of a hybrid vector which allows for temperature regulation of expression of cloned genes in cyanobacterium, Synechocystis 6803

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dc.contributor.advisor Vann, Carolyn N. en_US
dc.contributor.author Bae, Insoo en_US
dc.date.accessioned 2011-06-03T19:34:42Z
dc.date.available 2011-06-03T19:34:42Z
dc.date.created 1988 en_US
dc.date.issued 1988
dc.identifier LD2489.Z78 1988 .B3 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/183555
dc.description.abstract A hybrid vector, pBVl, has been constructed which is capable of regulating expression of cloned genes in both Escherichia coli and in cyanobacterium Synechocystis 6803. Vector pBVl contains the powerful rightward promoter of bacteriophage lambda to ensure that cloned genes are transcibed at a high level. In addition, pBV] also contains a gene, cI857, encoding the lambda temperature sensitive repressor protein.The CAT gene coding for chloramphenicol aceyltransferase (CmActase) was cloned into pBV1 downstream of the lambda regulatory features to make plasmid pTC1. The expression of the CAT gene was quantified spectrophotometrically following addition of chloramphenicol and a shift in the growth temperature of the cell culture. The specific activity of CmActase increased from 540 to 5400 units within 30 minutes. Thus, using the newly constructed hybrid vector, pBVl, temperature regulation of gene expression in E. coli was observed.
dc.description.sponsorship Department of Biology
dc.format.extent iii, 39 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Genetic vectors. en_US
dc.subject.lcsh Molecular cloning. en_US
dc.subject.lcsh Escherichia coli. en_US
dc.subject.lcsh Cyanobacteria. en_US
dc.subject.lcsh Genetic engineering. en_US
dc.title Construction of a hybrid vector which allows for temperature regulation of expression of cloned genes in cyanobacterium, Synechocystis 6803 en_US
dc.description.degree Thesis (M.S.)
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/544002 en_US


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  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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