The inability of amblyomma americanum adults to transmit borrelia burgdorferi

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dc.contributor.advisor Pinger, R. R. en_US Timmons, Lynette F. en_US 2011-06-03T19:36:46Z 2011-06-03T19:36:46Z 1994 en_US 1994
dc.identifier LD2489.Z78 1994 .T56 en_US
dc.description.abstract Uninfected nymphs of Ixodes scapularis and Amblyomma americanum were fed on hamsters that had been injected intraperitoneally with a 0.5 ml sample of Borrelia burgdorferi (2.5 X 10' spirochetes per ml) 30 days earlier. All nymphs fed to repletion and were then housed during the molting process. In order to assess their ability to transmit the spirochetes, the resulting l. scapularis and A. americanum adults were allowed to feed on uninfected rabbits.Dissection of the adult l. scapularis ticks revealed 9/12 (75%) to harbor motile spirochetes, identified as B. burgdorferi by darkfield microscopy, isolation in BSK II medium, and indirect immunofluorescent antibody staining with the monoclonal antibody H5332. Transmission was successful to one of two New Zealand White rabbits by these infected ticks.Dissection of the adult A. americanum ticks revealed 0/150 (0%) to harbor spirochetes. Transmission to each of three rabbits was unsuccessful. However, 5/90 (5.6%) cultures of midgut material from these same ticks, harbored non-motile spirochete-like bodies. The identity of these "spirochetes" is unknown.
dc.description.sponsorship Department of Biology
dc.format.extent vii, 89 leaves ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Lyme disease -- Transmission. en_US
dc.subject.lcsh Vector-pathogen relationships. en_US
dc.subject.lcsh Borrelia. en_US
dc.subject.lcsh Amblyomma. en_US
dc.subject.lcsh Ixodes. en_US
dc.title The inability of amblyomma americanum adults to transmit borrelia burgdorferi en_US Thesis (M.S.)
dc.identifier.cardcat-url en_US

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  • Master's Theses [5406]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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