Effects of dietary cyclopropene fatty acids on estrogen production in strain A/ST mice

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dc.contributor.advisor Bennett, Alice S. en_US
dc.contributor.author Cooper, David C. en_US
dc.date.accessioned 2011-06-03T19:37:33Z
dc.date.available 2011-06-03T19:37:33Z
dc.date.created 1996 en_US
dc.date.issued 1996
dc.identifier LD2489.Z78 1996 .C66 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/185661
dc.description.abstract Cyclopropene fatty acids (CPFA) found in cottonseed oil (CT) have been shown to reduce production of progesterone, a precursor of estrogen. Estrogenic hormones have been implicated in enhancing growth of mammary tumors. In this study, the effect of dietary cottonseed oil on estrogen production by mature female mice was determined by measuring urinary estrogen using High Performance Liquid Chromatography.At four months of age, five groups of three Strain A/ST female mice were placed on 20% fat diets containing 0, 2.5, 5.0, 10, and 20% cottonseed oil. The remainder of the fat in the experimental diets was corn oil sufficient to provide the balance of the 20% fat content in conjunction with other nutrients of equal percentages in all diets. At five day intervals mice were housed in metabolic cages and twenty-four hour urine samples were collected. Urine was purified on C18 columns and eluted with 1% phosphoric acid: acetonitrile: methanol: (54:35:11). Estrogen was quantified by High Performance Liquid Chromatography using a 250 X 5mm C18 column, hydrocortisone as an internal standard, and variable wavelength recorder set at 242 nm. The level of urinary estrogens after day 35 of the study was lower in all diets containing cottonseed oil. This is in agreement with several authors who have reported instances of physiological abnormalities in mammals which were fed increasing but low levels of dietary cyclopropenes. Since elevated estrogen levels have been identified as a risk for breast cancer, this study examines the relationship between dietary cyclopropenes and estrogen hormone production in strain A/St mice.
dc.description.sponsorship Department of Biology
dc.format.extent v, 54 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Fatty acids -- Physiological effect. en_US
dc.subject.lcsh Estrogen. en_US
dc.subject.lcsh Carcinogenesis. en_US
dc.title Effects of dietary cyclopropene fatty acids on estrogen production in strain A/ST mice en_US
dc.description.degree Thesis (M.S.)
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1014848 en_US

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  • Master's Theses [5358]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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