Isolation of differentially expressed messages in sexually reproducing tripsacum dactyloides

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dc.contributor.advisor Blakey, C. Ann en_US Houghteling, Billy B. en_US 2011-06-03T19:38:21Z 2011-06-03T19:38:21Z 1998 en_US 1998
dc.identifier LD2489.Z78 1998 .H68 en_US
dc.description.abstract The isolation and characterization of the gene(s) associated with and potentially responsible for the regulation of apomixis (asexual) and sexual reproduction in the grass species Tripsacum dactyloides is quintessential to agricultural advancement. Apomixis is the mechanism by which plants can produce seed without fertilization, where all progeny are genetically identical to the maternal parent. In natural populations of the genus Tripsacum, lower ploidy forms (i.e., diploid, 2n=36) reproduce sexually and the higher ploidy forms (triploid, 3n=54; tetraploid, 4n=72; etc.) reproduce asexually via apomixis. In order to gain a better understanding of sexual reproductive processes in plants, subtractive hybridization was performed on early and late female inflorescence gene products. This procedure allows for the recovery of gene products in the form of complimentary DNA, cDNA molecules, which correspond to messenger RNA (mRNA) present. These cDNA molecules were then used to extract unique sequence messages from the early developmental stage ovule tissue of diploid T. dactyloides. These cDNA molecules will allow for the direct isolation of the original form of the gene(s) from a large fragment library of the Tripsacum genome. Isolation and characterization of these gene(s) is of pivotal importance to our understanding of alternate modes of reproduction in Tripsacum.
dc.description.sponsorship Department of Biology
dc.format.extent vii, 93 leaves : ill. (some col.), map ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Tripsacum -- Genetic engineering. en_US
dc.subject.lcsh Tripsacum -- Reproduction. en_US
dc.subject.lcsh Plant hybridization. en_US
dc.subject.lcsh Apomixis. en_US
dc.title Isolation of differentially expressed messages in sexually reproducing tripsacum dactyloides en_US Thesis (M.S.)
dc.identifier.cardcat-url en_US

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  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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