Intracellular levels of reduced and oxidized glutathione in the tissue zones of the rat kidney

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dc.contributor.advisor Zamlauski-Tucker, Marianna J. en_US DeWitt, Jason A. en_US 2011-06-03T19:38:36Z 2011-06-03T19:38:36Z 1999 en_US 1999
dc.identifier LD2489.Z78 1999 .D49 en_US
dc.description.abstract The purpose of this study was to determine the levels of whole tissue, cytosolic, and mitochondrial glutathione in the tissue zones of the rat kidney. Reduced (GSH) and oxidized glutathione (GSSG) were measured spectrophotometrically in tissue homogenates. Differential centrifugation was used to isolate the cytosolic and mitochondrial fractions. Cortical GSH and GSSG levels accounted for 51% and 60%, respectively, of the GSH and GSSG levels in the whole kidney. Cytosolic GSH levels were similar in the cortex and medulla but lower in the papilla. Cytosolic GSSG levels were highest in the cortex and lowest in the medulla. Mitochondrial GSH and GSSG levels did not follow a pattern similar to that of the cytosol or whole tissue. The mitochondrial redox ratio (GSH/GSH + GSSG X 100) was significantly higher in the cortex (ie., 67%) than the medulla (ie., 39%). The cytosolic redox ratio showed an opposite relationship with the cortex (ie., 57%) being lower than the medulla (ie., 78%). This study demonstrates that there are differences in GSH levels, GSSG levels, and the redox ratio in the tissue zones of the rat kidney.
dc.description.sponsorship Department of Physiology and Health Science
dc.format.extent iv, 50 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Glutathione -- Sampling. en_US
dc.subject.lcsh Rats -- Digestive organs. en_US
dc.subject.lcsh Kidneys -- Histochemistry. en_US
dc.title Intracellular levels of reduced and oxidized glutathione in the tissue zones of the rat kidney en_US Thesis (M.S.)
dc.identifier.cardcat-url en_US

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  • Master's Theses [5589]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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