Characterization of phosphofructokinase-M gene expression in preimplantation mouse embryos through the use of competitive reverse transcription-polymerase chain reaction

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dc.contributor.advisor Chatot, Clare L. en_US
dc.contributor.author Gobbett, Troy A. en_US
dc.date.accessioned 2011-06-03T19:38:44Z
dc.date.available 2011-06-03T19:38:44Z
dc.date.created 1999 en_US
dc.date.issued 1999
dc.identifier LD2489.Z78 1999 .G63 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/186582
dc.description.abstract The preimplantation mouse embryo undergoes many metabolic changes as development proceeds. One major change is the switch from a pyruvate based metabolism, to a glucose based metabolism. The phosphofructokinase enzyme is the regulatory enzyme of glycolysis and is thought to be a major contributor in controlling the block to glycolysis in early preimplantation mouse embryos. This study was undertaken to construct a system that would allow detection of RNA for the highly glycolytically active subunit (muscletype) of the phosphofructokinase (PFK) enzyme. A muscle specific mutant PFK plasmid was generated to provide mutant internal control RNA. Using this internal control, initial reverse transcriptionpolymerase chain reaction data collected from early embryo stages suggest that the muscle type PFK subunit RNA is not expressed in the preimplantation mouse at the 1-cell or blastocyst stages. This result suggests that PFK activity detected at the later morula and blastocyst stages must be from either a different PFK subunit or a novel embryonic form of PFK.
dc.description.sponsorship Department of Biology
dc.format.extent vii, 70 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Mice -- Embryos -- Physiology. en_US
dc.subject.lcsh Mice -- Metabolism. en_US
dc.subject.lcsh Mice -- Development. en_US
dc.subject.lcsh Phosphofructokinase 1. en_US
dc.title Characterization of phosphofructokinase-M gene expression in preimplantation mouse embryos through the use of competitive reverse transcription-polymerase chain reaction en_US
dc.description.degree Thesis (M.S.)
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1133725 en_US


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  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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