The effects of ischemia-reperfusion injury on cytosolic and mitochondrial levels of glutathione in the rat kidney

Cardinal Scholar

Show simple item record

dc.contributor.advisor Zamlauski-Tucker, Marianna J. en_US
dc.contributor.author Becker, Bryan A. en_US
dc.date.accessioned 2011-06-03T19:39:20Z
dc.date.available 2011-06-03T19:39:20Z
dc.date.created 2001 en_US
dc.date.issued 2001
dc.identifier LD2489.Z78 2001 .B43 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/187065
dc.description.abstract This study was done to investigate the effect of ischemia-reperfusion injury on cytosolic and mitochondrial glutathione levels in the rat kidney. Glutathione is the main cellular defense against free radicals that are thought to cause ischemia-reperfusion injury. Right kidneys from anesthetized female Lewis rats (9-12 months old) were exposed to 60 minutes of ischemia followed by 0, 30, or 120 minutes of reperfusion. The kidneys were perfused with isotonic saline, harvested, homogenized, and separated into cytosolic and mitochondrial fractions by differential centrifugation. Reduced (GSH) and oxidized (GSSG) glutathione levels were measured spectrophotometrically. There were significant decreases in both the GSH levels and the % GSH/Total Glutathione in the cytosol and mitochondria of kidneys exposed to ischemia-reperfusion injury when compared to control kidneys. The glutathione levels in either the cytosol or mitochondria did not recover even after 120 minutes of reperfusion. This study demonstrates that 60 minutes of ischemia followed by 0, 30, or 120 minutes of reperfusion decreases both cytosolic and mitochondrial levels of glutathione in the rat kidney.
dc.description.sponsorship Department of Physiology and Health Science
dc.format.extent vii, 52 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Reperfusion injury. en_US
dc.subject.lcsh Glutathione. en_US
dc.title The effects of ischemia-reperfusion injury on cytosolic and mitochondrial levels of glutathione in the rat kidney en_US
dc.description.degree Thesis (M.S.)
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1204198 en_US


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

Show simple item record

Search Cardinal Scholar


Browse

My Account