Optimization of HPLC techniques for separation of oxidized phosphatidylcholines

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dc.contributor.advisor Pattison, Scott, 1947- en_US
dc.contributor.author Weddle, Carolyn A. en_US
dc.date.accessioned 2011-06-03T19:40:32Z
dc.date.available 2011-06-03T19:40:32Z
dc.date.created 2005 en_US
dc.date.issued 2005
dc.identifier LD2489.Z78 2005 .W43 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/187935
dc.description.abstract In cellular studies of patients with lipid related disorders such as mammary cancers, leukemia, and artheroscierosis, separation of molecular species of oxidized phosphatidylcholine (PC) can be an important assistance in research or diagnosis. Goals of this project were to optimize separation of oxidized and unoxidized PC molecular species in a single HPLC chromatogram followed by in line identification of hydroperoxides. Oxidized egg PC's were produced using UV light exposure in air. Separations were performed on an Ultrasphere ODS column and an Asahipak ODPVA column using a Waters 2695 system with photodiode array. The ODPVA column routinely gave 10 times larger plate numbers. Various mobile phase mixtures (methanol, acetonitrile, water) and gradients were tested. The optimum gradient on our system is (1) 5 minutes, 47 % methanol/40 % acetonitrile/13 water in a linear gradient to (2) 17 minutes, 49 % methanol/40 % acetonitrile/11 % water to (3) 18 minutes, 29 % methanol/60 % acetonitrile/11 % water linearly to (4) 50 minutes, 31 % methanol/60 % acetonitrile/9 % water continued isocratically to 110 minutes. Oxidized hydroperoxides were detected by fluorescence using a post column reaction with diphenyl-1 pyrenylphosphine (DPPP). Both iron (III) and pyridine were tested as catalysts for this reaction.
dc.description.sponsorship Department of Chemistry
dc.format.extent viii, 77 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Lecithin -- Oxidation -- Analysis. en_US
dc.subject.lcsh High performance liquid chromatography. en_US
dc.title Optimization of HPLC techniques for separation of oxidized phosphatidylcholines en_US
dc.title.alternative Optimization of high performance liquid chromatography techniques for separation of oxidized phosphatidylcholines en_US
dc.description.degree Thesis (M.S.)
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1319835 en_US

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  • Master's Theses [5370]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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