Assessment of genetic diversity in Asarum canadense L. using amplified fragment length polymorphism (AFLP)

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dc.contributor.advisor Ruch, Donald Gene, 1948- en_US Quadri, Asima en_US
dc.coverage.spatial n-us-in en_US 2011-06-03T19:41:16Z 2011-06-03T19:41:16Z 2007 en_US 2007
dc.identifier LD2489.Z78 2007 .Q33 en_US
dc.description.abstract Forest fragmentation poses a serious danger to population diversity in plants and animals by increasing species isolation, thus reducing the population size and genetic diversity. However, little information is available concerning how fragmentation impacts plant diversity. AFLP fingerprinting was used to assess genetic diversity within and between populations of Asarum canadense L. (Canadian Wild Ginger) across 11 different populations in East-Central Indiana. AFLP fingerprints using two primer pairs generated 51 distinct bands with an average of 25.5 bands per primer. Forty-eight low molecular weight distinct polymorphic bands were observed (50-200 bp range). The percentage of polymorphism was low (0-25%) indicating low levels of genetic diversity within each population studied. NTSYSpc Numerical Taxonomy Analysis Software generated aphenogram that revealed high levels of homologies within populations (75-100%), with individuals from the same population typically clustered. The genetic diversity between populations ranged from 10-50%. The populations from Jay, Randolph and Henry Counties clustered together exhibiting -54% homology, while populations from Mien, Madison, and Huntington counties shared approximately 64% homology. The populations from Adams, Blackford, Delaware, and Grant counties shared approximately 66% homology. However, within this last group Blackford and Delaware counties shared 90% homology. There were no apparent effects of the size of the forest fragments on the observed diversity measures. A possible relationship between genetic diversity and spatial distance was observed between populations moving from east to west. Possible reasons for this observation may be due to forest types, age of forests, climatic factors, soil types, and/or anthropogenic activities. Overall, the low level of average diversity within the populations strongly suggests that the individuals inhabiting isolated forests primarily propagate by asexual means.Ball State UniversityMuncie, IN 47306 en_US
dc.description.sponsorship Department of Biology en_US
dc.format.extent x, 49 leaves : ill. (some col.), maps ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Asarum canadense -- Variation -- Indiana. en_US
dc.subject.lcsh Fragmented landscapes -- Indiana. en_US
dc.title Assessment of genetic diversity in Asarum canadense L. using amplified fragment length polymorphism (AFLP) en_US Thesis (M.S.) en_US
dc.identifier.cardcat-url en_US

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  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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