Streptococcal mAb10F5 interacts with synaptic vesicles due to antiphospholipod activity

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dc.contributor.advisor Kelly-Worden, Marie L. en_US Ghassemifar, Sara en_US 2011-06-03T19:41:43Z 2011-06-03T19:41:43Z 2008 en_US 2008
dc.identifier LD2489.Z78 2008 .G49 en_US
dc.description.abstract Hypermetabolism, observed in Sydenham's chorea (SC); a complication of acute rheumatoid fever (ARF) involving binding of streptococcal M protein antibodies in the brain, may result from an increase in glutamate release. The interaction of mAb 1 OF5, a specific M protein antibody subtype, with brain proteins (e.g. Rabphilin-3A), synaptic vesicles (SVs) and synaptosomal fraction (SF) was examined. Rat brain slices immunostained with mAb l OF5 revealed an interaction with choroid plexus and elements appearing to be neuropils. Dot blotting demonstrated an interaction of mAb I OF5 with both SVs and SFs. Western blotting revealed a smear from mAb 10F5 against the SF fraction. However, both modified SVs and pure liposomes examined by fluorescent and confocal microscopy bound mAbl0F5 suggesting a direct interaction with phospholipids. ELISA demonstrated binding of mAb1OF5 with negatively charged phospholipids involved in antiphospholipid syndrome (APS). Hypermetabolism and binding at the choroid plexus is observed in SC and APS supporting the connection between these disorders.
dc.description.sponsorship Department of Physiology and Health Science
dc.format.extent viii, 55 leaves : ill. (some col.) ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Bacterial antigens -- Immunology. en_US
dc.subject.lcsh Streptococcus. en_US
dc.subject.lcsh Autoimmune diseases -- Pathogenesis. en_US
dc.subject.lcsh Antiphospholipid syndrome -- Pathogenesis. en_US
dc.title Streptococcal mAb10F5 interacts with synaptic vesicles due to antiphospholipod activity en_US Thesis (M.S.)
dc.identifier.cardcat-url en_US

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  • Master's Theses [5330]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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