Inhibition of CDC42 activity at the cell membrane prevents host cell invasion of Staphylococcus aureus

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dc.contributor.advisor McDowell, Susan A. en_US
dc.contributor.author Brown, Amy L. en_US
dc.date.accessioned 2011-06-03T19:41:47Z
dc.date.available 2011-06-03T19:41:47Z
dc.date.created 2008 en_US
dc.date.issued 2008
dc.identifier LD2489.Z78 2008 .B76 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/188475
dc.description.abstract Staphylococcus aureus infections have become a widespread problem. Simvastatin decreases S. aureus invasion. Simvastatin use reduces prenylation of target proteins, including CDC42. Prenylated CDC42 is active at the cell membrane. Our hypothesis is that CDC42 activity at the cell membrane is needed for endocytic S. aureus invasion. The prenylation site on CDC42 was deleted and mutant CDC42 (CDC42C5O7V/V5) was transfected into mammalian cells, which were exposed to S. aureus. Decreased bacterial infection of up to 90% was seen in cells stably expressing CDC42C507V/V5. Mammalian cells were treated with secramine A, an inhibitor of CDC42 activity, and exposed to S. aureus. Decreased bacterial invasion of 70% in these cells was seen. These findings suggest that CDC42 activity at the cell membrane is needed for S. aureus cell invasion. These findings increase understanding of the mechanism of S. aureus cell invasion and could be used to develop new treatment or prevention methods.
dc.description.sponsorship Department of Biology
dc.format.extent xi, 63 leaves : ill. (some col.) ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Rho GTPases -- Inhibitors. en_US
dc.subject.lcsh Cell membranes -- Physiology. en_US
dc.subject.lcsh Staphylococcus aureus infections -- Prevention. en_US
dc.title Inhibition of CDC42 activity at the cell membrane prevents host cell invasion of Staphylococcus aureus en_US
dc.title.alternative Inhibition of cell division cycle 42 activity at the cell membrane prevents host cell invasion of Staphylococcus aureus en_US
dc.description.degree Thesis (M.S.)
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1391473 en_US


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  • Master's Theses [5510]
    Master's theses submitted to the Graduate School by Ball State University master's degree candidates in partial fulfillment of degree requirements.

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