Plasma hemoglobin determination : two manual methods compared : an honors thesis (HONRS 499)

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dc.contributor.advisor Behforouz, Nancy en_US
dc.contributor.advisor Strahl, K. Sharlene Emerick, Anne M. en_US 2011-06-06T18:45:12Z 2011-06-06T18:45:12Z 1994 en_US 1994
dc.identifier.other A-164 en_US
dc.description.abstract Plasma hemoglobin levels are clinically significant in the determination of the amount of in vivo hemolysis. In this study, two manual methods for plasma hemoglobin were compared. The current method uses sodium carbonate to measure oxyhemoglobin at three wavelengths: 380 nm, 415 nm, and 450 nm. The second method, from SIGMA Diagnostics, uses 3,3',5,5'-tetramethylbenzidine in a peroxidase reaction; the absorbance is then read at 600 nm. In the peroxidase method from SIGMA Diagnostics, timing plays a very crucial role in obtaining accurate results. The peroxidase procedure from SIGMA Diagnostics is less time consuming than the current method and is therefore more advantageous. The peroxidase method proves to be the better method due to better linearity and accuracy.
dc.description.sponsorship Honors College
dc.format.extent 15 leaves : ill. ; 28 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Medicine. en_US
dc.title Plasma hemoglobin determination : two manual methods compared : an honors thesis (HONRS 499) en_US
dc.type Undergraduate senior honors thesis. Thesis (B.S.)
dc.identifier.cardcat-url en_US

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  • Undergraduate Honors Theses [5928]
    Honors theses submitted to the Honors College by Ball State University undergraduate students in partial fulfillment of degree requirements.

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