Refinement of a HPLC assay of lipid hydroperoxides caused by free radical-mediated oxidation of membrane lipids : an honors thesis (HONRS 499)

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dc.contributor.advisor Pattison, Scott, 1947- en_US
dc.contributor.author Kenkel, Laura E. en_US
dc.date.accessioned 2011-06-06T19:03:53Z
dc.date.available 2011-06-06T19:03:53Z
dc.date.created 2004 en_US
dc.date.issued 2004
dc.identifier.other A-286 en_US
dc.identifier.uri http://cardinalscholar.bsu.edu/handle/handle/191120
dc.description.abstract A sensitive and easy-to-use HPLC assay that measures the amount of free radicalmediated oxidation of membrane lipids is refined. A reaction that models the effects of free radical oxidation on cell membrane lipids is used. The rate of the reaction between diphenyl-l-pyrenylphosphine (DPPP) and tert-butylhydroperoxide (tBuOOH; a model compound for peroxidized lipids) is detected by measuring the amount of fluorescent DPPPO. The levels of two catalysts, pyridine and iron (III), are adjusted so that the reaction they catalyze is complete within approximately one minute, with a goal of extrapolating back to reaction conditions that would be conducive to completion within one second. Samples of the reacting solution are injected into an HPLC machine, bypassing the column in favor of a spectrofluorometer.
dc.description.sponsorship Honors College
dc.format.extent 23 leaves : ill. ; 30 cm. en_US
dc.source Virtual Press en_US
dc.subject.lcsh Chemistry. en_US
dc.title Refinement of a HPLC assay of lipid hydroperoxides caused by free radical-mediated oxidation of membrane lipids : an honors thesis (HONRS 499) en_US
dc.type Undergraduate senior honors thesis.
dc.description.degree Thesis (B.?.)
dc.identifier.cardcat-url http://liblink.bsu.edu/catkey/1289995 en_US


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  • Undergraduate Honors Theses [5928]
    Honors theses submitted to the Honors College by Ball State University undergraduate students in partial fulfillment of degree requirements.

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