Crude carbon compounds were evaluated in several stages to determine the optimal liquid-culture medium for conidial production in Colletotrichum graminicola. Among those tested, use of unfiltered V8 medium resulted in optimum conidiation. % v/v of this medium was evaluated along with pH and conductivity using a central composite design to optimize conidiation in C. graminicola. The predicted titer using this model was 4.78x10 conidialmL, using a medium consisting of 40.14% v/v V8, with a pH of 6.16 and a conductivity of 28.03mS.