PURPOSE: Prolonged sitting has been shown to negatively impact various cardiometabolic and hemostatic variables, and intermittent exercise breaking up prolonged sitting appears to mitigate those changes. Therefore, the purpose of this study was to determine changes in fibrinolytic capacity in prolonged sitting broken up by low intensity exercise compared to uninterrupted sitting. METHODS: Nine apparently healthy adults (5 men, 4 women: 23.4±0.9 years) reported to the laboratory for two separate visits. They underwent two hours of uninterrupted sitting or two-hours of accumulated sitting broken up by low intensity exercise every 30 minutes. Blood was drawn to assess fibrinolytic responses via tissue plasminogen activator (t-PA), plasminogen activator inhibitor (PAI-1). RESULTS: There was a significant time-by-trial interaction (p=0.047, η2 =0.41) for PAI-1 activity. Additionally, a trend of time-by-trial interaction (p=0.06, η2 =0.37) was observed for t-PA antigen. CONCLUSION: Following two hours of sitting, fibrinolytic potential was diminished, however those changes were mitigated by interrupting the bout of sitting with brief periods of walking.