Effect of knocking out YLR419W on S. cerevisiae growth following proteotoxic, heat, osmotic, and oxidative stress

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Sharlow, Meredith
Smaldino, Philip J.
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Thesis (B.?)
Honors College
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YLR419W is a yeast homolog for DHX36, a gene which encodes a G-quadruplex helicase, in humans. By studying how knocking out YLR419W affects Saccharomyces cerevisiae growth and survival following cellular stress, we can learn more about the role of the protein Ylr419w in in responding to cellular stress. I hypothesized that when YLR419W is deleted, S. cerevisiae will be less able to survive stress. To test this hypothesis, growth and survival under proteotoxic, osmotic, oxidative, and heat induced stress was tested using yeast growth assays. Deletion of YLR419W in experimental clone 1 moderately inhibited growth under proteotoxic stress, and did not inhibit growth under thermal stress, osmotic stress, and oxidative stress. However, the proteotoxic stress effects were not repeatable across yeast clone replicates, suggesting that Ylr419w is not required for the proteotoxic stress response.