Candida viswanathii is a fungus that efficiently produces long-chain dicarboxylic acids, which are useful in petrochemical production. It is closely related to Candida tropicalis, a prominent human fungal pathogen. Because CRISPR has been implemented in other Candida species, particularly Candida albicans, I hypothesized that a similar CRISPR-mediated genome editing method could be developed for C. viswanathii. I have sequenced the C. viswanathii genome and identified guide sequences for ADE2 and CYP52. I then cloned these sequences into a vector that also expresses the Cas9 nuclease. I cotransformed these vectors along with a repair template that will introduce stop codons when incorporated into ADE2 and CYP52 of C. viswanathii. I was able to obtain many transformants, and I am now determining if these transformants have incorporated the repair template. My ultimate goal is to work in collaboration to determine why and how C. viswanathii makes long-chain dicarboxylic acids