A comparative analysis of the fatty acid composition of selected ascomycetes

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Kruger, Robert M., 1940-
Bennett, Alice S.
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Thesis (M.S.)--Ball State University, 1974.
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In this investigation an analytical study of the distribution and characteristics of the lipids and fatty acids of Aspergillus niger and Penicillium chrysogenum was made utilizing gas-liquid chromatography, thin-layer chromatography, and periodate-permanganate degradation.Triglycerides and free fatty acids were the most abundant lipid classes in 32 hour cultures of both species. In A. niger, total lipid of dry weight varied from 2.7% to 6,1% while P. chrysogenum ranged from 1.1% to 2,2%. The per cent water in the wet mycelium varied from 97.1% to 97.7i% for A. niger and ranged from 94.4% to 95,1% in P. chrysogenum.In A. niger, saturated fatty acids from octanoic (C8) to behenic (C22) were found with palmitic (C16) being the most abundant. No cyclopropane structures were found on the saturated acids present, Monoenoic acids from dodecenoic acid (C12:1) through oleic acid (C18:1) were found. Linoleic and linolenic acids were the only dienoic and trienoic acids present. Similar patterns were found in h. chrysogenum. In all cultures linoleic (Cl8:2) was more abundant than either oleic (C18:1) or linolenic (C18:3)Periodate-permanganate oxidation of oleic acid extracted from A. niger gave products indicating that the double bond was in the 9 position, Linoleic acid appeared to have double bonds in the 9 and 12 positions while double bonds in linolenic acid occupied the 6, 9 and 12 positions.Ball State UniversityMuncie, IN 47306