The effect of exposure to ozone on energy metabolism in heart, liver, and skeletal muscle tissues

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Mcallister, Richard Murray
Costill, David L.
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Thesis (M.A.)
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Energy Metabolism in Heart, Previous studies have demonstrated that acute or chronic exposureto ozone results in (1) alterations in the glutathione peroxidase system, and (2) the functional activity of sulfhydryls and sulfhydryl-contain+nq enzymes in lung tissue and erythrocytes. As a result, both structural damage and changes in metabolic profile occur. The present study examined tie effects of acute exposure to ozone on the metabolic properties of hamster heart(H), liver(L), and gastrocnemius(G) tissues. Measures included in vitro oxidative capacity(Q02) and the optimal activity of the marker enzymes 2-oxoglutarate Dehydrogenase(20GDII), Carnitine Palmitoyltransferase(CPT), hexokinase(HI;), Glucose-6-Phosphate Dehydrogenase(G6PDH), and Lactate Dehydrogenase(LDH). Relative to controls, both L and G tissues had significantlydepressed QC02 values(Pc0.01). Heart was unaffected. Significant treatment effects were also found for 20GDH(H,L), CPT(L), HK(H), and G6PDH(H). The data support previous work on lung and erythrocytes suggesting shifts in metabolic profiles in an attempt to counteract the deleterious effects of ozone exposure on cellular lipids or enzymes with sulfhydryl groups. Thepresent data also indicate that brief exposure to ozone will have an effect upon tissues other than the lungs or erythrocytes.