Carotid Artery Pulsatility Index (PI) is a non-invasive method of assessing vascular resistance. It is calculated from the difference between peak-systolic velocity and end-diastolic velocity divided by the mean flow velocity. Excessive pulsatility within the cerebrovasculature gradually reduces elastic recoil via arterial stiffening which in turn, diminishes pulsatile damping. This pulsatile stress is associated with microvascular lesions and has emerged as a probable contributor to cardiovascular and cerebrovascular disease. The research purpose was to test the reproducibility of measuring PI in the common carotid artery in order to establish this technique in the Clinical Exercise Physiology Program. This study demonstrated minimal variance in measurements between visits, supporting reproducibility of PI for prospective research.