Effects of sulfuric-nitric acid solutions on mortality and development of Aedes triseriatus larvae and pupae

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Fadely, Keith R.
Siewert, Horst F.
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Thesis (M.S.)
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Larvae of Ae. triseriatus mosquitoes were collected from treeholes on the Ball State University campus. These insects were raised to adulthood under laboratory conditions. Newly hatched larvae were raised in deionized water. One hundred twenty-five of instar 1 mosquitoes (group A) were placed into separate baby food jars containing deionized water. These jars were separated into five groups. The pH of the water in each group was adjusted to 3, 4, 5, 6 and 7 with a 70% to 30% sulfuric-nitric acid solution. Subsequent groups consisting of instar 2, 3 and 4 larvae and pupae (groups B, C, D and E respectively) were exposed to the same experimental treatment as that of group A. The effects of each of these pH levels on mortality and development was observed until the insects reached maturity or died.Larvae in water of pH 3 experienced a higher percentage of mortality than mosquitoes exposed to less acidic solutions. A decrease in mortality with age was observed. Mortality of insects in group A was greater than that of any other group. Mosquitoes in group D experienced the lowest mortality.Variations of developmental time for mosquitoes in pH 3 were probably due to high mortality. Developmental time was similar for all groups. Slight variations between these groups may have been due to natural fluctuations in development. The total average developmental time for Ae. triseriatus mosquitoes of this study was 11.12 days.