It is well known that pulse pressure (PP) is a predictor of cardiovascular disease. However, there are inconclusive results concerning the association between PP and allcause mortality. Additionally, there has been no direct research assessing the influence of sex and age on the association between PP and all-cause mortality. PURPOSE: To assess sex differences in the relationship between resting PP and all-cause mortality in adults 18 years and older. METHODS: Participants were 3,911 apparently healthy adults (2,211 men and 1,700 women) from the Ball State Adult Fitness Longitudinal Lifestyle STudy (BALL ST) cohort that performed a comprehensive health screening and a maximal cardiopulmonary maximal exercise test to measure VO2max. Participants were divided into groups by sex and age (<55 yrs and ≥55 yrs) and followed for 25.7±12.2 yrs for all-cause mortality. Blood pressure (BP) measurements were used to calculate PP by calculating the difference between systolic BP and diastolic BP. Coxproportional hazard models were performed to determine the association between PP and all-cause mortality. RESULTS: PP was associated with all-cause mortality in men and women (p<0.05). When adjusted for age, sex, test year, traditional risk factors, and VO2max, PP only remained significant in men. After dividing men and women into young (<55 yrs) and old (≥55 yrs) groups, PP only remained significant in young men. CONCLUSION: PP was a predictor of all-cause mortality in men and women. However, PP was not independent of traditional risk factors for women. Additionally, PP was independent of traditional risk factors including VO2max for young males (<55 yrs) but not old males (≥55 yrs). These findings support the clinical value of PP. Thus, clinicians should consider utilizing PP as a routine clinical measurement.