Extraction and concentration of macrophages from murine lymphnodes : [an honors thesis (HONRS 499)]

Abstract

This project involved the development of a protocol for releasing culturing adherent macrophages from murine 1ymph nodes. The process was based on methods used in extracting macrophage cells from the red pulp of spleens and liver granulomas using collagenase treatment. Some data on macrophage la expression in mouse models of leishmaniasis was collected in experiments leading to the development the Protocol.